Key words : Chicken microsatellites, Cross-species amplification, Anas platyrhynchos, Genetic diversity.Ĭhromosome homology is highly conserved between chicken (Gallus gallus) and Japanese quail (Coturnix japonica) and a few chromosome rearrangements occurred in the evolution of these two species. The results provide evidences of the applicability of chicken microsatellite markers in determining the genetic variations and relationship among three ducks populations in India. Dendrogram based on Nei’s genetic distance grouped Assam and West Bengal duck populations and separated the Uttarakhand duck population. Eleven loci confirmed Hardy-Weinberg equilibrium (P>0.05) and no evidence for linkage disequilibrium was observed among pairs of loci. The polymorphic information content (PIC) ranged from 0.43 to 0.92 with an average of 0.68. The observed heterozygosity across populations ranged from between 0.15 and 0.91, with mean (± SE) of 0.63 ± 0.21, while the expected heterozygosity ranged 0.45 to 0.94 with mean (± SE) of 0.72 ± 0.13. Observed and effective number of alleles ranged from 4 to 21 and 1.80 to 13.34, respectively. Twenty three of the 30 loci were amplified and 17 loci yielded high success rate (> 91%). We obtained blood samples of 57 Indian ducks ( Anas platyrhynchos ) belonging to three indigenous duck populations of geographically distinct locations of the country and genotyped them using chicken microsatellite markers. KeywordsChicken microsatellites–Cross-species amplification–Himalayan monal– Loci could be employed in population genetic studies for Himalayan monal and their applicability in Jungle Bush Quail, Grey Our study show that these 15 microsatellites No evidence for linkage disequilibrium was observed among pair of loci. While 12 loci conformed to Hardy–Weinberg equilibrium (P > 0.05), 3 loci, i.e. Heterozygosity in the population ranged between 0.285 and 0.714, with mean (± s.e.) of 0.499 ± 0.125, indicating considerable All the testedġ5 microsatellite markers were polymorphic, with mean (± s.e.) allelic number of 4 ± 1.51, ranging 2–7 per locus. Fifteen microsatellite markers developed for chicken were cross-amplified in Himalayan monal. We have reported microsatellite loci for Himalayan monal for Microsatellite loci for closely related taxa in bird species. Therefore, cross-species microsatellite amplification can be an effective way in obtaining Isolation and development of new microsatellite markers for any species is still labour-intensive and requires substantial
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